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Fig. 3A. —55-year-old man after above-knee amputation for chronic vascular disease. MR image obtained with surface perpendicular to main magnetic field (B0, black arrow) shows three-layer appearance. High-signal-intensity transitional layer (white arrow) is present. Because surface is curved, influence of orientation is apparent. Increased signal intensity in deep layer and decreased signal intensity in superficial regions of transitional layer of cartilage are evident where sample is not perpendicular to B0.