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Fig. 1A. —35-year-old healthy male volunteer in whom placement of measurement slice and determination of region of interest is illustrated. Measurement slice is indicated on midsagittal localizer image. It is perpendicular to localizer image and passes through junction of anterior three quarters and posterior quarter of vertebral body L4. Slight angulation from true coronal plane is applied to make slice pass optimally through posterior parts of L3 and L5.