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Fig. 4B. —74-year-old woman with moderately differentiated 2-cm hepatocellular carcinoma (HCC) showing weak vascular endothelial growth factor (VEGF) expression in right inferior segment of liver with chronic type C viral hepatitis. Child-Pugh grade was A. Schematic shows electrophoretic bands and their corresponding histograms. For calibration, 1.25 mg/mL of VEGF solution was used. Areas of histogram were 480 pixels for calibration band, 761 pixels for HCC band, and 2,375 pixels for surrounding liver band. VEGF expression index was 1.59 in HCC and 4.95 in surrounding liver, giving difference in VEGF expression index of –3.36. Note that electrophoretic peak adjacent to that of liver is due to expression of irregular protein.