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Fig. 5B. —64-year-old man with well-differentiated 4.6-cm hepatocellular carcinoma (HCC) showing weak vascular endothelial growth factor (VEGF) expression in right posteroinferior segment of liver with chronic type C viral hepatitis. Child-Pugh grade was B. Schematic shows electrophoretic bands and their corresponding histograms. For calibration, 1.25 mg/mL of VEGF solution was used. Areas of histogram were 902 pixels for calibration band, 1,101 pixels for HCC band, and 1,638 pixels for surrounding liver band. VEGF expression index was 1.22 in HCC and 1.82 in surrounding liver, giving difference in VEGF expression index of –0.60. Note that electrophoretic peaks adjacent to those of HCC and liver are due to expression of irregular protein.