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Fig. 6B. —77-year-old woman with moderately differentiated 6.8-cm hepatocellular carcinoma (HCC) showing moderate vascular endothelial growth factor (VEGF) expression in lateral segment of liver with chronic type C viral hepatitis. Child-Pugh grade was A. Schematic shows electrophoretic bands and their corresponding histograms. For calibration, 1.25 mg/mL of VEGF solution was used. Areas of histogram were 312 pixels for calibration band, 1,654 pixels for HCC band, and 738 pixels for surrounding liver band. VEGF expression index was 5.30 in HCC and 2.37 in surrounding liver, giving difference in VEGF expression index of 2.93. Note that electrophoretic peak adjacent to that of liver is due to expression of irregular protein.