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Fig. 1F —Group A: swine sacrificed immediately after radiofrequency ablation. Photograph of nicotinamide adenine dinucleotide (NADH) diaphorase-stained section of tissue presented in E shows outermost layer observed on H and E-stained frozen section is found on border of NADH diaphorase-stained lesions. In its internal regions (E), not-NADH-stained lesion conforms to ablated lesion, which is same as coagulation necrosis. However, outermost layer contains admixture of stained and not-stained cells (arrows). (NADH diaphorase, x20)