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Fig. 4A —Correlation between diameter-based and region-of-interest (ROI)-based methods. Scattergrams of volume measurement derived with diameter-based (x-axis) and ROI-based (y-axis) methods are shown at four measurement time points, at radiation therapy (RT) start (A), at 20-25 Gy/2-2.5 weeks (B), at 45-50 Gy/4-5 weeks (C), and post-RT (follow-up at 1-2 months) (D). Measurements derived by the two methods correlate well before (A) and after (D) RT (r = 0.89 and r = 0.88, respectively) but poorly during RT at 2-2.5 weeks (B) and at 4-5 weeks (C) (r = 0.68 and r = 0.67, respectively). Poor correlation during therapy is likely related to increasing irregularity of tumor volumes that are still sufficiently large during therapy to have impacted correlation. Later, post-RT, as tumor volume decreased further, impact of tumor irregularity is small in magnitude and correlation of diameter-based with 3D ROI-based measurement improves.