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Fig. 4A —Photomicrographs of resected gallbladder immediately after radiofrequency ablation with parallel direction in group with 0.5-cm distance from gallbladder wall (group Ai [immediate] and group Ap [parallel]). Destruction of whole layer (arrows, A and B and red circle, A) of gallbladder wall and replacement with necrotic tissue in gallbladder wall are shown on low-power view (A) (H and E, x12.5) and high power view (B) (H and E, x40). Arrowheads and black circle represent viable muscle layer of gallbladder wall.