PATTERNS OF PROTEIN SYNTHESIS IN THE HELA CANCER CELL

¹ Advanced Academic Fellow of the James Picker Foundation; Postdoctoral Fellow of the Department of Biology, Massachusetts Institute of Technology; and Clinical Fellow of the Department of Radiology, Massachusetts General Hospital

Variations in radiosensitivity occur during the cell cycle. In order to better understand the reasons for these variations, protein synthesis during the cell cycle has been studied. Proteins synthesized in one stage of the cell cycle have been labeled with one isotope and those proteins synthesized during other stages of the cell cycle labeled with a second isotope. The proteins so labeled were co-electrophoresed on polyacrylamide gels. The radioactivity distributions on the gels were analyzed and showed significant differences in the distributions of the two isotopes. Such differences indicate differences in the kinds of and/or rates of synthesis of proteins as the cell goes through the cell cycle. No definite correlation can yet be made between specific proteins or protein synthetic patterns and specific cell cycle changes in radiosensitivity. However, the fact that changes in the patterns of protein synthesis are here shown to exist suggests that further study of patterns of protein synthesis may lead to basic mechanisms of radiosensitivity.

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