EARLY MACROMOLECULAR CHANGES AFTER RELEASE OF CONTACT INHIBITION BY THE GLASS BEAD METHOD

¹ Advanced Academic Fellow of the James Picker Foundation.

An understanding of cellular radiation effects requires knowledge of the mechanisms responsible for cell division and its initiation. Tissue culture cells can be brought to a resting state and then induced to divide in synchronous fashion by use of the glass bead method. The release of contact inhibition and subsequent initiation of cell division is unaccompanied by any large switch-on of gene transcription, implying that only subtle changes actually trigger cell division. These changes in 3T3 cells require some messenger RNA synthesis in G₁ before DNA synthesis begins. In 3T6 cells, contact inhibited either just before or in the midst of S, new messenger RNA synthesis after release of contact inhibition is unnecessary for these cells to proceed through S, but is necessary for them to traverse G₂.

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