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Postmortem Angiography After Vascular Perfusion with Diesel Oil and a Lipophilic Contrast Agent

¹ Institute of Forensic Medicine, University of Bern, IRM, Buehlstrasse 20, CH-3012 Bern, Switzerland.
² Institute of Anatomy, University of Bern, Bern, Switzerland.
³ Institute of Veterinary Anatomy, University of Bern, Bern, Switzerland.
⁴ CT/MRI-Center, Graz, Austria.
⁵ Institute of Diagnostic Radiology, University of Bern, Bern, Switzerland.

OBJECTIVE. The objective of our study was to establish optimal perfusion conditions for high-resolution postmortem angiography that would permit dynamic visualization of the arterial and venous systems.

MATERIALS AND METHODS. Cadavers of two dogs and one cat were perfused with diesel oil through a peristaltic pump. The lipophilic contrast agent Lipiodol Ultra Fluide was then injected, and angiography was performed. The efficiency of perfusion was evaluated in the chick chorioallantoic membrane.

RESULTS. Vessels could be seen up to the level of the smaller supplying and draining vessels. Hence, both the arterial and the venous sides of the vascular system could be distinguished. The chorioallantoic membrane assay revealed that diesel oil enters microvessels up to 50 µm in diameter and that it does not penetrate the capillary network.

CONCLUSION. After establishing a postmortem circulation by diesel oil perfusion, angiography can be performed by injection of Lipiodol Ultra Fluide. The resolution of the images obtained up to 3 days after death is comparable to that achieved in clinical angiography.

Keywords: angiography • animal studies • contrast media • noninvasive autopsy • postmortem angiography • virtopsy • virtual autopsy

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				S. Grabherr, V. Djonov, K. Yen, M. J. Thali, and R. Dirnhofer Postmortem Angiography: Review of Former and Current Methods Am. J. Roentgenol., March 1, 2007; 188(3): 832 - 838. [Abstract] [Full Text] [PDF]