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Fig. 4B. —Proteomic analysis of spreading depression–induced
changes in neocortex of mouse 3 days after spreading depression.
Polyacrylamide one-dimensional electrophoresis gel of right (experimental)
cortex after fractionization 3 days after spreading depression. At least 56
upregulated or newly expressed protein bands in experimental hemisphere are
shown. Mass analysis matrix-assisted laser desorption/ionization
time-of-flight mass spectroscopy identified half of upregulated proteins,
which include extra- and intracellular structural components, metabolic
proteins, kinases, and other miscellaneous gene products: (4) collagen alpha
chain precursor, (5) clathrin heavy chain, (12) T complex protein delta, (15)
vacuolar adenosine triphosphate synthetase subunit A, (17) syntaxin-binding
protein, (21) tubulin, (22) calcium-calmodulin-dependent protein kinase type
II, (23) tubulin, (24) phosphoglycerate kinase, (25) actin, (27) fructose
bisphosphonate aldolase, (31) glyceraldehyde-3-phosphate dehydrogenase, (32)
malate dehydrogenase (syntaxin 1A), (36) vacuolar adenosine triphosphate
synthetase subunit E, (40) glutathione S-transferase P1, (41) superoxide
dismutase precursor, (44) cofilin, (46) peptidyl-prolyl cis-trans
isomerase, (47) heat shock protein 70, (49) phosphatidylinositol transfer
protein alpha isoform, (50) F-actin capping protein alpha isoform, and (53)
creatine kinase.
