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Fig. 3E —Phantoms of various numbers of ferumoxide-poly-L-lysine
(PLL) labeled adipose-derived stem cells implanted in agar in Petri dish,
which was placed on top of 3.0-cm-diameter radiofrequency surface coil. Series
of multislice T2-weighted and T2*-weighted 7-T MR images (TR/TE,
250/10-40 in increments of 5 milliseconds; matrix size, 128 x 128; field
of view, 4.0 cm; slice thickness, 1 mm) were acquired with standard spin-echo
sequences. T2-weighted images reveal clear differences in signal intensity
between area without cells (A) and areas with increasing number of
superparamagnetic iron oxide (SPIO)-labeled adipose-derived stem cells.
Numbers of SPIO-labeled adipose-derived stem cells correlated well with
decrease in MR signal intensity. Low signal intensity at all cell numbers
greater than 10 cells suggests MRI can depict at least as few as 10 labeled
adipose-derived stem cells with nominal pixels. Number of cells, 1,000.
